Green Synthesis for the Production of Glucose Syrup from Waste Cassava Starch Using Alpha-Amylase

  • Fatai A. Aderibigbe University of Ilorin, Ilorin, Nigeria
  • Esther O. Babatunde University of Ilorin, Ilorin, Nigeria
  • Sunday O. Ochapa University of Ilorin, Ilorin, Nigeria
  • Harvis B. Saka University of Ilorin, Ilorin, Nigeria
Keywords: starch, silver nanoparticles, alpha-amylase, hydrolysis

Abstract

Research on production of glucose syrup from waste cassava starch has not been adequately covered. In this paper, we report on the process for breaking down starch through harnessing the catalytic properties of silver nanoparticles (AgNPs) in an enzymatic procedure that encompasses the breakdown of starch by alpha-amylase. The analysis carried out on the starch-synthesized AgNPs had its peak absorbance in the region between 400 nm and 500 nm, the X-ray diffraction (XRD) result also showed that the AgNPs were crystalline and this indicated that the nanoparticle formed could be used for hydrolysis. The presence of silver nanoparticles was observed to enhance the speed at which starch was enzymatically broken down as sample G1 (which was the sample with an equal amount of silver nanoparticle and alpha-amylase) has a concentration of 5.23 mg/mL (which implies a percentage conversion of approximately 52.3%). It is hypothesized that alpha-amylase became immobilized on the nanoparticle surface giving rise to a better glucose yield in this study.

Author Biographies

Esther O. Babatunde, University of Ilorin, Ilorin, Nigeria
Esther Oluwabunmi Babatunde is a Lecturer at the University of Ilorin, Department of Chemical Engineering, Nigeria. She holds a Bachelor's degree (LAUTECH Ogbomoso, 2006), a Master of Engineering (F.U.T. Minna, 2015), and a PhD (F.U.T. Minna, 2021) in Chemical Engineering. Her research areas are renewable energy, catalysis, waste management, seed oil utilization, and Biochemical Engineering. She has few publications in biofuel production, reaction and kinetics studies as well as biochemical Engineering and catalysis. She has supervised undergraduate students’ research projects.
Sunday O. Ochapa, University of Ilorin, Ilorin, Nigeria
Sunday Onazi Ochapa holds a B.Sc in Chemical Engineering from the University of Ilorin. His research interests are Biochemical Engineering, Bioenergy, and Waste Management
Harvis B. Saka, University of Ilorin, Ilorin, Nigeria
Harvis Bamidele Saka obtained his first degree in Chemical Engineering (B.Tech) at the Ladoke Akintola University of Technology, Ogbomosho Oyo state, Nigeria (2015). 2019 he also obtained a Master of Engineering in Chemical Engineering (M.Eng) at the University of Ilorin, Ilorin, and Kwara State. He has published original articles in high-impact journals in the area of his interest. His research interests include biofuel, lubricants, bioproducts, wastewater treatment, carbon capture technology, biomass conversion, and catalysis. He is currently on his PhD research.

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Published
2024-07-01